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The supervisor oversees a given shift at a Crime Lab.
- Gil Grissom - Season 1-9, night shift supervisor
- Catherine Willows - Season 9-11, 16-, night shift supervisor
- D.B. Russell - Season 12-16, night shift supervisor
- Catherine Willows - Season 5, swing shift supervisor
- Conrad Ecklie - Season 1-5, day shift supervisor
- Sofia Curtis - Season 5, acting day shift supervisor
- Sara Sidle - Season 11.05, day shift supervisor
- Megan Donner - pre-Season 1, day shift supervisor
- Horatio Caine - Season 1-10, swing day Shift supervisor
- Mac Taylor - Season 1-9, day shift supervisor